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In the context of the recent advances in human reproductive technology, “Pronuclear Transfer” is used for

  1. Fertilization of egg in Vitro by the donor sperm
  2. Genetic modification of sperm-producing cells
  3. Development of stem cells into functional embryos
  4. Prevention of mitochondrial diseases in offspring

Explanation

Option (d) is correct
  • Pro-nuclear transfer is a technique used to prevent the transmission of mitochondrial diseases from mother to child. This process involves transferring the nuclear DNA from a fertilized egg with defective mitochondria into a donor egg with healthy mitochondria. The resulting embryo, therefore, has healthy mitochondria while retaining the genetic material from the parents, thus reducing the risk of mitochondrial disorders.

    Diagram explaining mitochondrial donation therapy as an advanced fertility method to prevent mitochondrial disorders from mother to child. It illustrates three-parent baby technique using mother's egg with faulty mitochondria, donor egg with healthy mitochondria, and father's sperm, highlighting steps like removal of faulty mitochondria, separation of nucleus, IVF process, and resulting embryo with healthy mitochondria, along with key techniques such as Maternal Spindle Transfer and Pronuclear Transfer.

Answer: (d) Prevention of mitochondrial diseases in offspirng; Difficulty Level: Hard

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